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CISD1 Monoclonal antibody

CISD1 Monoclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, chicken, rabbit, pig

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D7E3

Cat No : 68030-1-Ig

Print datasheet

Synonyms

C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1

验证数据展示

  • WB analysis using 68030-1-Ig

    WB analysis using 68030-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68030-1-Ig (CISD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 68030-1-Ig

    IHC staining of human pancreas cancer using 68030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68030-1-Ig (CISD1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of H9C2 using 68030-1-Ig

    IF Staining of H9C2 using 68030-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CISD1 antibody (68030-1-Ig, Clone: 1D7E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, pig brain tissue, rabbit brain tissue, rat brian tissue, mouse brain tissue, chicken brain tissue, mouse cerebellum tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inH9C2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68030-1-Ig targets CISD1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, chicken, rabbit, pig samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat, chicken, rabbit, pig
Immunogen CISD1 fusion protein Ag8560 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CDGSH iron sulfur domain 1
Synonyms C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1
Calculated Molecular Weight 108 aa, 12 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC007043
Gene Symbol CISD1
Gene ID (NCBI) 55847
RRIDAB_2918772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZ45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

实验方案

Product Specific Protocols
WB protocol for CISD1 antibody 68030-1-IgDownload protocol
IHC protocol for CISD1 antibody 68030-1-IgDownload protocol
IF protocol for CISD1 antibody 68030-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols