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验证数据展示

  • WB analysis using 68030-1-Ig (same clone as 68030-1-PBS)

    WB analysis using 68030-1-Ig (same clone as 68030-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68030-1-Ig (CISD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 68030-1-Ig (same clone as 68030-1-PBS)

    IHC staining of human pancreas cancer using 68030-1-Ig (same clone as 68030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68030-1-Ig (CISD1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 68030-1-Ig (same clone as 68030-1-PBS)

    IF Staining of H9C2 using 68030-1-Ig (same clone as 68030-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using CISD1 antibody (68030-1-Ig, Clone: 1D7E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

CISD1 Monoclonal antibody, PBS Only

CISD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68030-1-PBS

产品说明书

CloneNo. 1D7E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit, chicken

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:2.6.1.3, Cysteine transaminase CISD1, CDGSH iron-sulfur domain-containing protein 1, CDGSH iron sulfur domain 1, C10orf70

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68030-1-PBS targets CISD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit, chicken
免疫原 CISD1 fusion protein Ag8560 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CDGSH iron sulfur domain 1
别名 EC:2.6.1.3, Cysteine transaminase CISD1, CDGSH iron-sulfur domain-containing protein 1, CDGSH iron sulfur domain 1, C10orf70
计算分子量 108 aa, 12 kDa
观测分子量 14-17 kDa
GenBank蛋白编号BC007043
基因名称 CISD1
Gene ID (NCBI) 55847
RRIDAB_2918772
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NZ45
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.