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CISD1 Monoclonal antibody, PBS Only

CISD1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, chicken, rabbit, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D7E3

Cat No : 68030-1-PBS

Print datasheet

Synonyms

C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1

验证数据展示

  • WB analysis using 68030-1-Ig (same clone as 68030-1-PBS)

    WB analysis using 68030-1-Ig (same clone as 68030-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68030-1-Ig (CISD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 68030-1-Ig (same clone as 68030-1-PBS)

    IHC staining of human pancreas cancer using 68030-1-Ig (same clone as 68030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68030-1-Ig (CISD1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 68030-1-Ig (same clone as 68030-1-PBS)

    IF Staining of H9C2 using 68030-1-Ig (same clone as 68030-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CISD1 antibody (68030-1-Ig, Clone: 1D7E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

产品信息

68030-1-PBS targets CISD1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, chicken, rabbit, pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, chicken, rabbit, pig
Immunogen CISD1 fusion protein Ag8560 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CDGSH iron sulfur domain 1
Synonyms C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1
Calculated Molecular Weight 108 aa, 12 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC007043
Gene Symbol CISD1
Gene ID (NCBI) 55847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZ45
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.