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CD35 Monoclonal antibody

CD35 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B8E6

Cat no : 68033-1-Ig

Print datasheet

Synonyms

KN, CR1, Complement receptor type 1, C3BR, C3b/C4b receptor

验证数据展示

  • IHC staining of human tonsillitis using 68033-1-Ig

    IHC staining of human tonsillitis using 68033-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68033-1-Ig (CD35 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CR1/CD35 antibody included
  • IHC staining of human tonsillitis using 68033-1-Ig

    IHC staining of human tonsillitis using 68033-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68033-1-Ig (CD35 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CR1/CD35 antibody included
  • IF Staining of human appendicitis using 68033-1-Ig

    IF Staining of human appendicitis using 68033-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD35 antibody (68033-1-Ig, Clone: 1B8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human appendicitis using 68033-1-Ig

    IF Staining of human appendicitis using 68033-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CR1 antibody (68033-1-Ig, Clone: 1B8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:550-1:2200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68033-1-Ig targets CD35 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen CD35 fusion protein Ag30268 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Synonyms KN, CR1, Complement receptor type 1, C3BR, C3b/C4b receptor
Calculated Molecular Weight 224 kDa
GenBank Accession NumberNM_000573
Gene Symbol CR1
Gene ID (NCBI) 1378
ENSEMBL Gene IDENSG00000203710
RRIDAB_2918775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17927
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes. A soluble form of CD35 has been found in plasma (PMID: 3156931). It has been reported that soluble CD35 is produced by a proteolytic cleavage in the C-terminal region of CD35 transmembrane domain (PMID: 9405292).

实验方案

Product Specific Protocols
IHC protocol for CD35 antibody 68033-1-IgDownload protocol
IF protocol for CD35 antibody 68033-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols