IHCeasy CR1/CD35 Ready-To-Use IHC Kit
CR1/CD35 Ready-to-use reagent kit for IHC.
Reactivity
Human
Sample Type
FFPE tissue
Cat No : KHC0102
Synonyms
C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN
Validation Data Gallery
Product Information
KHC0102 is a ready-to-use IHC kit for staining of CR1/CD35. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy CR1/CD35 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Complement receptor type 1 (CR1), also known as C3b/C4b receptor or CD35, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains. CR1 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhan cells and glomerular podocytes. CR1 acts as a receptor for C3b and C4b and plays important roles in regulation of the complement cascade and clearance of immune complexes. A soluble form of CR1 (sCR1) has been found in plasma. It has been reported that sCR1 is produced by a proteolytic cleavage in the C-terminal region of CR1 transmembrane domain.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN |