验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, human placenta tissue, LNCaP cells, THP-1 cells, Jurkat cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68034-1-Ig targets ADPGK in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | ADPGK fusion protein Ag31242 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ADP-dependent glucokinase |
| 别名 | 2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35 |
| 计算分子量 | 497 aa, 54 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC006112 |
| 基因名称 | ADPGK |
| Gene ID (NCBI) | 83440 |
| RRID | AB_2918776 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9BRR6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADPGK antibody 68034-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |