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ADPGK Monoclonal antibody

ADPGK Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B4B8

Cat no : 68034-1-Ig

Print datasheet

Synonyms

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35

验证数据展示

  • WB analysis using 68034-1-Ig

    WB analysis using 68034-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of LNCaP using 68034-1-Ig

    WB analysis of LNCaP using 68034-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 68034-1-Ig

    WB analysis of A549 using 68034-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68034-1-Ig

    WB analysis of HeLa using 68034-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 68034-1-Ig

    WB analysis of THP-1 using 68034-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 68034-1-Ig

    WB analysis of human placenta using 68034-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, human placenta tissue, LNCaP cells, THP-1 cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68034-1-Ig targets ADPGK in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen ADPGK fusion protein Ag31242 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADP-dependent glucokinase
Synonyms 2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35
Calculated Molecular Weight 497 aa, 54 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC006112
Gene Symbol ADPGK
Gene ID (NCBI) 83440
RRIDAB_2918776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

实验方案

Product Specific Protocols
WB protocol for ADPGK antibody 68034-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols