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ADPGK Monoclonal antibody, PBS Only

ADPGK Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B4B8

Cat no : 68034-1-PBS

Print datasheet

Synonyms

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35

验证数据展示

  • WB analysis using 68034-1-Ig (same clone as 68034-1-PBS)

    WB analysis using 68034-1-Ig (same clone as 68034-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 68034-1-Ig (same clone as 68034-1-PBS)

    WB analysis of LNCaP using 68034-1-Ig (same clone as 68034-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 68034-1-Ig (same clone as 68034-1-PBS)

    WB analysis of A549 using 68034-1-Ig (same clone as 68034-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68034-1-Ig (same clone as 68034-1-PBS)

    WB analysis of HeLa using 68034-1-Ig (same clone as 68034-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 68034-1-Ig (same clone as 68034-1-PBS)

    WB analysis of THP-1 using 68034-1-Ig (same clone as 68034-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68034-1-Ig (same clone as 68034-1-PBS)

    WB analysis of human placenta using 68034-1-Ig (same clone as 68034-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

产品信息

68034-1-PBS targets ADPGK in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ADPGK fusion protein Ag31242 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADP-dependent glucokinase
Synonyms 2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35
Calculated Molecular Weight 497 aa, 54 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC006112
Gene Symbol ADPGK
Gene ID (NCBI) 83440
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRR6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).