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验证数据展示

  • WB analysis using 68051-1-Ig

    WB analysis using 68051-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of mouse testis using 68051-1-Ig

    IHC staining of mouse testis using 68051-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GAS2L1 Monoclonal antibody

GAS2L1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68051-1-Ig

产品说明书

CloneNo. 2C6D5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

GAR22, GAS2 like protein 1, GAS2L1, growth arrest 2 like 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, mouse testis tissue, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68051-1-Ig targets GAS2L1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 GAS2L1 fusion protein Ag31232 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 growth arrest-specific 2 like 1
别名 GAR22, GAS2 like protein 1, GAS2L1, growth arrest 2 like 1
计算分子量 73 kDa
观测分子量73 kDa
GenBank蛋白编号BC011047
基因名称 GAS2L1
Gene ID (NCBI) 10634
RRIDAB_2918793
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99501
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAS2L1(growth arrest‐specific 2‐like 1) is a component of the cytoskeleton that mediates the interaction between microtubules and microfilament (PMID: 34555082). GAS2L1 contains an N‐terminal actin‐binding calponin homology (CH) domain, a MT lattice‐binding Gas2‐related (GAR) domain and a C‐terminal SxIP motif, which mediates the interaction with MT plus ends via EB proteins. GAS2L1 regulates microtubule dynamics and stability by interacting with microtubule plus-end tracking proteins, such as MAPRE1, to regulate microtubule growth along actin stress fibers (PubMed:24706950).

实验方案

Product Specific Protocols
WB protocol for GAS2L1 antibody 68051-1-IgDownload protocol
IHC protocol for GAS2L1 antibody 68051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols