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验证数据展示

  • WB analysis using 68066-1-Ig

    WB analysis using 68066-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of mouse testis using 68066-1-Ig

    IHC staining of mouse testis using 68066-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68066-1-Ig (NDUFS3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFS3 antibody included

NDUFS3 Monoclonal antibody

NDUFS3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68066-1-Ig

产品说明书

CloneNo. 1A1B3

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, rabbit, mouse, rat

应用

WB, IHC, ELISA

别名

CI 30kD, Complex I 30kD, NDUFS3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NDUFS3 Ready-To-Use IHC Kit. NDUFS3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68066-1-Ig targets NDUFS3 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, pig, rabbit, mouse, rat
免疫原 NDUFS3 fusion protein Ag7072 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
别名 CI 30kD, Complex I 30kD, NDUFS3
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC000617
基因名称 NDUFS3
Gene ID (NCBI) 4722
RRIDAB_2918807
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75489
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.

实验方案

Product Specific Protocols
WB protocol for NDUFS3 antibody 68066-1-IgDownload protocol
IHC protocol for NDUFS3 antibody 68066-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols