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NDUFS3 Monoclonal antibody, PBS Only

NDUFS3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1B3

Cat no : 68066-1-PBS

Print datasheet

Synonyms

CI 30kD, Complex I 30kD, NDUFS3

验证数据展示

  • WB analysis using 68066-1-Ig (same clone as 68066-1-PBS)

    WB analysis using 68066-1-Ig (same clone as 68066-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68066-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 68066-1-Ig (same clone as 68066-1-PBS)

    IHC staining of mouse testis using 68066-1-Ig (same clone as 68066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68066-1-Ig (NDUFS3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68066-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFS3 antibody included
Planning an IHC experiment? We recommend our IHCeasy NDUFS3 Ready-To-Use IHC Kit. NDUFS3 primary antibody included.

产品信息

68066-1-PBS targets NDUFS3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rabbit, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, pig, rabbit, mouse, rat
Immunogen NDUFS3 fusion protein Ag7072 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
Synonyms CI 30kD, Complex I 30kD, NDUFS3
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC000617
Gene Symbol NDUFS3
Gene ID (NCBI) 4722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75489
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.