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NDUFS3 Monoclonal antibody

NDUFS3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1B3

Cat no : 68066-1-Ig

Print datasheet

Synonyms

CI 30kD, Complex I 30kD, NDUFS3

验证数据展示

  • WB analysis using 68066-1-Ig

    WB analysis using 68066-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of mouse testis using 68066-1-Ig

    IHC staining of mouse testis using 68066-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68066-1-Ig (NDUFS3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFS3 antibody included

经过测试的应用

Positive WB detected inA549 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NDUFS3 Ready-To-Use IHC Kit. NDUFS3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68066-1-Ig targets NDUFS3 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, pig, rabbit, mouse, rat
Immunogen NDUFS3 fusion protein Ag7072 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
Synonyms CI 30kD, Complex I 30kD, NDUFS3
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC000617
Gene Symbol NDUFS3
Gene ID (NCBI) 4722
RRIDAB_2918807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75489
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.

实验方案

Product Specific Protocols
WB protocol for NDUFS3 antibody 68066-1-IgDownload protocol
IHC protocol for NDUFS3 antibody 68066-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols