• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68075-1-Ig

    WB analysis using 68075-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of Jurkat using 68075-1-Ig

    FC experiment of Jurkat using 68075-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Septin 5 Monoclonal antibody (68075-1-Ig, Clone:1E2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Septin 5 Monoclonal antibody

Septin 5 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68075-1-Ig

产品说明书

CloneNo. 1E2A3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, FC (Intra), ELISA

别名

SEPT5, Cell division control-related protein 1, CDCrel-1, CDCREL1, CDCREL 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inpig brain tissue, LNCaP cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68075-1-Ig targets Septin 5 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Septin 5 fusion protein Ag2205 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 septin 5
别名 SEPT5, Cell division control-related protein 1, CDCrel-1, CDCREL1, CDCREL 1
计算分子量 369 aa, 43 kDa
观测分子量40-43 kDa
GenBank蛋白编号BC025261
基因名称 Septin 5
Gene ID (NCBI) 5413
RRIDAB_2918813
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99719
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.

实验方案

Product Specific Protocols
WB protocol for Septin 5 antibody 68075-1-IgDownload protocol
FC protocol for Septin 5 antibody 68075-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols