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验证数据展示

  • WB analysis of human testis using 68081-1-Ig

    WB analysis of human testis using 68081-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 68081-1-Ig

    IHC staining of human kidney using 68081-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 68081-1-Ig

    WB analysis of HepG2 using 68081-1-Ig

    WB result of DPEP1 antibody (68081-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPEP1 transfected HepG2 cells.

  • IF Staining of mouse kidney using 68081-1-Ig

    IF Staining of mouse kidney using 68081-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DPEP1 antibody (68081-1-Ig, Clone: 2B4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

DPEP1 Monoclonal antibody

DPEP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68081-1-Ig

产品说明书

CloneNo. 2B4F4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, ELISA

别名

hRDP, EC:3.5.2.6, EC:3.4.13.19, Dehydropeptidase-I, Beta-lactamase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, rabbit kidney tissue, pig pancreas tissue, rat pancreas tissue, HepG2 cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68081-1-Ig targets DPEP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 DPEP1 fusion protein Ag14591 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 dipeptidase 1 (renal)
别名 hRDP, EC:3.5.2.6, EC:3.4.13.19, Dehydropeptidase-I, Beta-lactamase
计算分子量 411 aa, 46 kDa
观测分子量46 kDa
GenBank蛋白编号BC017023
基因名称 DPEP1
Gene ID (NCBI) 1800
RRIDAB_2918818
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16444
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DPEP1 antibody 68081-1-IgDownload protocol
IHC protocol for DPEP1 antibody 68081-1-IgDownload protocol
IF protocol for DPEP1 antibody 68081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols