验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, rabbit kidney tissue, HEK293 cells, LNCaP cells, MCF-7 cells, U2OS cells |
| Positive IF/ICC detected in | U2OS cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
68087-1-Ig targets PYCRL in WB, IF/ICC, ELISA applications and shows reactivity with human, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rabbit |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag31938 Product name: Recombinant human PYCRL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 136-274 aa of BC007993 Sequence: IVMARGRHVGSSETKLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | pyrroline-5-carboxylate reductase-like |
| 别名 | PYCR3, EC:1.5.1.2, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase 3 |
| 计算分子量 | 274 aa, 29 kDa |
| 观测分子量 | 26-29 kDa |
| GenBank蛋白编号 | BC007993 |
| 基因名称 | PYCRL |
| Gene ID (NCBI) | 65263 |
| RRID | AB_2918824 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q53H96 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PYCRL antibody 68087-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
