• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PYCRL Monoclonal antibody

PYCRL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rabbit and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G7A3

Cat no : 68087-1-Ig

Print datasheet

Synonyms

FLJ13852, PYCRL

验证数据展示

  • WB analysis using 68087-1-Ig

    WB analysis using 68087-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68087-1-Ig (PYCRL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rabbit kidney using 68087-1-Ig

    WB analysis of rabbit kidney using 68087-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68087-1-Ig (PYCRL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, rabbit kidney tissue, HEK293 cells, LNCaP cells, MCF-7 cells, U2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68087-1-Ig targets PYCRL in WB, ELISA applications and shows reactivity with Human, Rabbit samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Rabbit
Cited Reactivityhuman
Immunogen PYCRL fusion protein Ag31938 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pyrroline-5-carboxylate reductase-like
Synonyms FLJ13852, PYCRL
Calculated Molecular Weight 274 aa, 29 kDa
Observed Molecular Weight26-29 kDa
GenBank Accession NumberBC007993
Gene Symbol PYCRL
Gene ID (NCBI) 65263
RRIDAB_2918824
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ53H96
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PYCRL antibody 68087-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Discov

Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer

Authors - Tina Becirovic
  • KD Validated
View Article