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验证数据展示

  • IF Staining of PC-3 using CL488-68087

    IF Staining of PC-3 using CL488-68087

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 PYCRL antibody (CL488-68087, Clone: 1G7A3 ) at dilution of 1:200.

  • IF Staining of U2OS using CL488-68087

    IF Staining of U2OS using CL488-68087

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 PYCRL antibody (CL488-68087, Clone: 1G7A3 ) at dilution of 1:200.

  • FC experiment of HEK-293T using CL488-68087

    FC experiment of HEK-293T using CL488-68087

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PYCRL (CL488-68087, Clone:1G7A3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated PYCRL Monoclonal antibody

PYCRL Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68087

产品说明书

CloneNo. 1G7A3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

PYCR3, EC:1.5.1.2, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase 3

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells, PC-3 cells
Positive FC (Intra) detected inHEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 68087-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68087 targets PYCRL in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag31938

Product name: Recombinant human PYCRL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 136-274 aa of BC007993

Sequence: IVMARGRHVGSSETKLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pyrroline-5-carboxylate reductase-like
别名 PYCR3, EC:1.5.1.2, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase 3
计算分子量 274 aa, 29 kDa
观测分子量26-29 kDa
GenBank蛋白编号BC007993
基因名称 PYCRL
Gene ID (NCBI) 65263
RRIDAB_2934620
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ53H96
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PYCRL antibody CL488-68087Download protocol
Standard Protocols
Click here to view our Standard Protocols
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