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CoraLite® Plus 488-conjugated PYCRL Monoclonal antibody

PYCRL Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G7A3

Cat no : CL488-68087

Print datasheet

Synonyms

PYCR3, Pyrroline-5-carboxylate reductase-like protein, Pyrroline-5-carboxylate reductase 3, P5CR 3, P5C reductase 3

验证数据展示

  • FC experiment of HEK-293T using CL488-68087

    FC experiment of HEK-293T using CL488-68087

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PYCRL (CL488-68087, Clone:1G7A3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 68087-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68087 targets PYCRL in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen PYCRL fusion protein Ag31938 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pyrroline-5-carboxylate reductase-like
Synonyms PYCR3, Pyrroline-5-carboxylate reductase-like protein, Pyrroline-5-carboxylate reductase 3, P5CR 3, P5C reductase 3
Calculated Molecular Weight 274 aa, 29 kDa
Observed Molecular Weight26-29 kDa
GenBank Accession NumberBC007993
Gene Symbol PYCRL
Gene ID (NCBI) 65263
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ53H96
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PYCRL antibody CL488-68087Download protocol
Standard Protocols
Click here to view our Standard Protocols