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  • Featured Product
  • KD/KO Validated

REEP5 Monoclonal antibody, PBS Only (Capture)

REEP5 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H8D8

Cat no : 68119-1-PBS

Print datasheet

Synonyms

Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1, C5orf18

验证数据展示

  • WB analysis using 68119-1-Ig (same clone as 68119-1-PBS)

    WB analysis using 68119-1-Ig (same clone as 68119-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68119-1-Ig (same clone as 68119-1-PBS)

    WB analysis of HeLa using 68119-1-Ig (same clone as 68119-1-PBS)

    WB result of REEP5 antibody (68119-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 68119-1-Ig (same clone as 68119-1-PBS)

    IHC staining of human colon cancer using 68119-1-Ig (same clone as 68119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 68119-1-Ig (same clone as 68119-1-PBS)

    IF Staining of A549 using 68119-1-Ig (same clone as 68119-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (68119-1-Ig, Clone: 1H8D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • WB analysis of pig heart using 68119-1-Ig (same clone as 68119-1-PBS)

    WB analysis of pig heart using 68119-1-Ig (same clone as 68119-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50780-1

    Cytometric bead array standard curve of MP50780-1

    Cytometric bead array standard curve of MP50780-1, REEP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68119-1-PBS. Detection antibody: 68119-2-PBS. Standard:Ag6229. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50780-2

    Cytometric bead array standard curve of MP50780-2

    Cytometric bead array standard curve of MP50780-2, REEP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68119-1-PBS. Detection antibody: 68119-3-PBS. Standard:Ag6229. Range: 0.098-100 ng/mL.

产品信息

68119-1-PBS targets REEP5 as part of a matched antibody pair:

MP50780-1: 68119-1-PBS capture and 68119-2-PBS detection (validated in Cytometric bead array)

MP50780-2: 68119-1-PBS capture and 68119-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen REEP5 fusion protein Ag6229 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name receptor accessory protein 5
Synonyms Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1, C5orf18
Calculated Molecular Weight 21 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC065926
Gene Symbol REEP5
Gene ID (NCBI) 7905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00765
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).