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REEP5 Monoclonal antibody

REEP5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H8D8

Cat no : 68119-1-Ig

Print datasheet

Synonyms

C5orf18, D5S346, DP1, Polyposis locus protein 1, Protein TB2, receptor accessory protein 5, REEP5, TB2

验证数据展示

  • WB analysis using 68119-1-Ig

    WB analysis using 68119-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HeLa using 68119-1-Ig

    WB analysis of HeLa using 68119-1-Ig

    WB result of REEP5 antibody (68119-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells.

  • IHC staining of human colon cancer using 68119-1-Ig

    IHC staining of human colon cancer using 68119-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 68119-1-Ig

    IF Staining of A549 using 68119-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (68119-1-Ig, Clone: 1H8D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • WB analysis of pig heart using 68119-1-Ig

    WB analysis of pig heart using 68119-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, pig heart tissue, HeLa cells, LNCaP cells, Jurkat cells, MOLT-4 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68119-1-Ig targets REEP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, pig
Immunogen REEP5 fusion protein Ag6229 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name receptor accessory protein 5
Synonyms C5orf18, D5S346, DP1, Polyposis locus protein 1, Protein TB2, receptor accessory protein 5, REEP5, TB2
Calculated Molecular Weight 21 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC065926
Gene Symbol REEP5
Gene ID (NCBI) 7905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00765
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

实验方案

Product Specific Protocols
WB protocol for REEP5 antibody 68119-1-IgDownload protocol
IHC protocol for REEP5 antibody 68119-1-IgDownload protocol
IF protocol for REEP5 antibody 68119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols