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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated REEP5 Monoclonal antibody

REEP5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8D8

Cat no : CL488-68119

Print datasheet

Synonyms

Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1, C5orf18

验证数据展示

  • IF Staining of A549 using CL488-68119

    IF Staining of A549 using CL488-68119

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 REEP5 antibody (CL488-68119, Clone: 1H8D8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68119

    FC experiment of HeLa using CL488-68119

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human REEP5 (CL488-68119, Clone:1H8D8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68119-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68119 targets REEP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, pig
Immunogen REEP5 fusion protein Ag6229 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name receptor accessory protein 5
Synonyms Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1, C5orf18
Calculated Molecular Weight 21 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC065926
Gene Symbol REEP5
Gene ID (NCBI) 7905
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ00765
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 REEP5 antibody CL488-68119Download protocol
Standard Protocols
Click here to view our Standard Protocols