PFKP Monoclonal antibody
PFKP Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Rabbit and More (2)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1H4D4
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, A549 cells, MCF-7 cells, HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PFKP Ready-To-Use IHC Kit. PFKP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
68129-1-Ig targets PFKP in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, Mouse, Rat, Rabbit |
| Cited Reactivity | human, mouse |
| Immunogen | PFKP fusion protein Ag25206 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | phosphofructokinase, platelet |
| Synonyms | 6 phosphofructokinase type C, FLJ40226, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase |
| Calculated Molecular Weight | 784 aa, 86 kDa |
| Observed Molecular Weight | 80-86 kDa |
| GenBank Accession Number | BC029138 |
| Gene Symbol | PFKP |
| Gene ID (NCBI) | 5214 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q01813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PFKP antibody 68129-1-Ig | Download protocol |
| IF protocol for PFKP antibody 68129-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
medRxiv Depressed Proximal Glycolysis in Myocardium Of Human Heart Failure with Preserved Ejection Fraction | ||
Metabolism Protein kinase N1 deficiency results in upregulation of cerebral energy metabolism and is highly protective in in vivo and in vitro stroke models |
