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验证数据展示

  • IF Staining of HeLa using CL488-68129

    IF Staining of HeLa using CL488-68129

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PFKP antibody (CL488-68129, Clone: 1H4D4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PFKP Monoclonal antibody

PFKP Monoclonal Antibody for IF/ICC
Cat No. CL488-68129

产品说明书

CloneNo. 1H4D4

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Rabbit

应用

IF/ICC

别名

6 phosphofructokinase type C, FLJ40226, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PFKP Ready-To-Use IHC Kit. PFKP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68129-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68129 targets PFKP in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat, Rabbit
免疫原 PFKP fusion protein Ag25206 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphofructokinase, platelet
别名 6 phosphofructokinase type C, FLJ40226, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase
计算分子量 784 aa, 86 kDa
观测分子量80-86 kDa
GenBank蛋白编号BC029138
基因名称 PFKP
Gene ID (NCBI) 5214
RRIDAB_3084410
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ01813
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PFKP antibody CL488-68129Download protocol
Standard Protocols
Click here to view our Standard Protocols