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验证数据展示

  • WB analysis using 68133-1-Ig

    WB analysis using 68133-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68133-1-Ig

    IHC staining of human lung cancer using 68133-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 68133-1-Ig

    WB analysis of HeLa using 68133-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68133-1-Ig

    WB analysis of NIH/3T3 using 68133-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 68133-1-Ig

    WB analysis of A549 using 68133-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68133-1-Ig

    IHC staining of human lung cancer using 68133-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 68133-1-Ig

    IF Staining of HeLa using 68133-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLA2G4A antibody (68133-1-Ig, Clone: 2D10H8 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PLA2G4A Monoclonal antibody

PLA2G4A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68133-1-Ig

产品说明书

CloneNo. 2D10H8
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells, MJ cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68133-1-Ig targets PLA2G4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse
文献引用反应性human
免疫原 PLA2G4A fusion protein Ag32674 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase A2, group IVA (cytosolic, calcium-dependent)
别名 cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A
计算分子量 749 aa, 85 kDa
观测分子量85-100 kDa
GenBank蛋白编号BC114340
基因名称 PLA2G4A
Gene ID (NCBI) 5321
RRIDAB_2923660
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP47712
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165).

实验方案

Product Specific Protocols
WB protocol for PLA2G4A antibody 68133-1-IgDownload protocol
IHC protocol for PLA2G4A antibody 68133-1-IgDownload protocol
IF protocol for PLA2G4A antibody 68133-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FASEB J

Mechano-induced arachidonic acid metabolism promotes keratinocyte proliferation through cPLA2 activity regulation

Authors - Shengzhou Shan
View Article