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PLA2G4A Monoclonal antibody, PBS Only

PLA2G4A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D10H8

Cat no : 68133-1-PBS

Print datasheet

Synonyms

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A

验证数据展示

  • WB analysis using 68133-1-Ig (same clone as 68133-1-PBS)

    WB analysis using 68133-1-Ig (same clone as 68133-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    WB analysis of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 68133-1-Ig (same clone as 68133-1-PBS)

    WB analysis of NIH/3T3 using 68133-1-Ig (same clone as 68133-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 68133-1-Ig (same clone as 68133-1-PBS)

    WB analysis of A549 using 68133-1-Ig (same clone as 68133-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    IF Staining of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLA2G4A antibody (68133-1-Ig, Clone: 2D10H8 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

产品信息

68133-1-PBS targets PLA2G4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen PLA2G4A fusion protein Ag32674 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Synonyms cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight85-100 kDa
GenBank Accession NumberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47712
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C