验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells, MJ cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
68133-1-Ig targets PLA2G4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, mouse |
Cited Reactivity | human |
Immunogen | PLA2G4A fusion protein Ag32674 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | phospholipase A2, group IVA (cytosolic, calcium-dependent) |
Synonyms | cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A |
Calculated Molecular Weight | 749 aa, 85 kDa |
Observed Molecular Weight | 85-100 kDa |
GenBank Accession Number | BC114340 |
Gene Symbol | PLA2G4A |
Gene ID (NCBI) | 5321 |
RRID | AB_2923660 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P47712 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165).
实验方案
Product Specific Protocols | |
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WB protocol for PLA2G4A antibody 68133-1-Ig | Download protocol |
IHC protocol for PLA2G4A antibody 68133-1-Ig | Download protocol |
IF protocol for PLA2G4A antibody 68133-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |