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ARPC2 Monoclonal antibody

ARPC2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D8E3

Cat no : 68145-1-Ig

Print datasheet

Synonyms

p34-ARC, p34 Arc, ARC34, Actin-related protein 2/3 complex subunit 2, 1D8E3

验证数据展示

  • WB analysis using 68145-1-Ig

    WB analysis using 68145-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68145-1-Ig (ARPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • FC experiment of HeLa using 68145-1-Ig

    FC experiment of HeLa using 68145-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (68145-1-Ig, Clone:1D8E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human stomach cancer using 68145-1-Ig

    IHC staining of human stomach cancer using 68145-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC2 antibody included
  • IHC staining of human stomach cancer using 68145-1-Ig

    IHC staining of human stomach cancer using 68145-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC2 antibody included
  • IF Staining of HeLa using 68145-1-Ig

    IF Staining of HeLa using 68145-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ARPC2 antibody (68145-1-Ig, Clone: 1D8E3 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, human peripheral blood platelets, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARPC2 Ready-To-Use IHC Kit. ARPC2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68145-1-Ig targets ARPC2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen ARPC2 fusion protein Ag7082 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin related protein 2/3 complex, subunit 2, 34kDa
Synonyms p34-ARC, p34 Arc, ARC34, Actin-related protein 2/3 complex subunit 2, 1D8E3
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC000590
Gene Symbol ARPC2
Gene ID (NCBI) 10109
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15144
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARPC2 antibody 68145-1-IgDownload protocol
IHC protocol for ARPC2 antibody 68145-1-IgDownload protocol
IF protocol for ARPC2 antibody 68145-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols