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验证数据展示

  • FC experiment of HeLa using CL488-68145

    FC experiment of HeLa using CL488-68145

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ARPC2 (CL488-68145, Clone:1D8E3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-68145

    IF Staining of HeLa using CL488-68145

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ARPC2 antibody (CL488-68145, Clone: 1D8E3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ARPC2 Monoclonal antibody

ARPC2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68145

产品说明书

CloneNo. 1D8E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

p34-ARC, p34 Arc, ARC34, Actin-related protein 2/3 complex subunit 2, 1D8E3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARPC2 Ready-To-Use IHC Kit. ARPC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68145-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68145 targets ARPC2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 ARPC2 fusion protein Ag7082 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 actin related protein 2/3 complex, subunit 2, 34kDa
别名 p34-ARC, p34 Arc, ARC34, Actin-related protein 2/3 complex subunit 2, 1D8E3
计算分子量 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC000590
基因名称 ARPC2
Gene ID (NCBI) 10109
RRIDAB_3084416
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15144
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ARPC2 antibody CL488-68145Download protocol
FC protocol for CL Plus 488 ARPC2 antibody CL488-68145Download protocol
Standard Protocols
Click here to view our Standard Protocols