验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, chicken heart tissue, HeLa cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68150-1-Ig targets NDUFV2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig, Chicken samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse, Rat, Rabbit, Pig, Chicken |
| 文献引用反应性 | rat |
| 免疫原 | NDUFV2 fusion protein Ag7760 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa |
| 别名 | NDUFV2 |
| 计算分子量 | 27 kDa |
| 观测分子量 | 24-27 kDa |
| GenBank蛋白编号 | BC001632 |
| 基因名称 | NDUFV2 |
| Gene ID (NCBI) | 4729 |
| RRID | AB_2923674 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P19404 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH: ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFV2 antibody 68150-1-Ig | Download protocol |
| IHC protocol for NDUFV2 antibody 68150-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
