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验证数据展示

  • WB analysis using 68150-1-Ig (same clone as 68150-1-PBS)

    WB analysis using 68150-1-Ig (same clone as 68150-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • WB analysis of chicken heart using 68150-1-Ig (same clone as 68150-1-PBS)

    WB analysis of chicken heart using 68150-1-Ig (same clone as 68150-1-PBS)

    chicken heart tissue were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

NDUFV2 Monoclonal antibody, PBS Only

NDUFV2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68150-1-PBS

产品说明书

CloneNo. 3B4A5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Rabbit, Pig, Chicken

应用

WB, IHC, Indirect ELISA

别名

NDUFV2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68150-1-PBS targets NDUFV2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig, Chicken samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Rabbit, Pig, Chicken
免疫原 NDUFV2 fusion protein Ag7760 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
别名 NDUFV2
计算分子量 27 kDa
观测分子量24-27 kDa
GenBank蛋白编号BC001632
基因名称 NDUFV2
Gene ID (NCBI) 4729
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP19404
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH: ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).