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NDUFV2 Monoclonal antibody, PBS Only

NDUFV2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig, Chicken

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B4A5

Cat no : 68150-1-PBS

Print datasheet

Synonyms

NDUFV2

验证数据展示

  • WB analysis using 68150-1-Ig (same clone as 68150-1-PBS)

    WB analysis using 68150-1-Ig (same clone as 68150-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • WB analysis of chicken heart using 68150-1-Ig (same clone as 68150-1-PBS)

    WB analysis of chicken heart using 68150-1-Ig (same clone as 68150-1-PBS)

    chicken heart tissue were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

产品信息

68150-1-PBS targets NDUFV2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig, Chicken samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Pig, Chicken
Immunogen NDUFV2 fusion protein Ag7760 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
Synonyms NDUFV2
Calculated Molecular Weight 27 kDa
Observed Molecular Weight24-27 kDa
GenBank Accession NumberBC001632
Gene Symbol NDUFV2
Gene ID (NCBI) 4729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19404
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH: ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).