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验证数据展示

  • WB analysis using 68153-1-Ig

    WB analysis using 68153-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68153-1-Ig

    WB analysis using 68153-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68153-1-Ig

    WB analysis of HepG2 using 68153-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 68153-1-Ig

    IF Staining of A431 using 68153-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AIDA antibody (68153-1-Ig, Clone: 1D11A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

AIDA Monoclonal antibody

AIDA Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68153-1-Ig

产品说明书

CloneNo. 1D11A1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, HepG2 cells, HT-29 cells, U2OS cells, K-562 cells
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68153-1-Ig targets AIDA in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 AIDA fusion protein Ag20597 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 axin interactor, dorsalization associated
别名 AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7
计算分子量 306 aa, 35 kDa
观测分子量35 kDa
GenBank蛋白编号BC015535
基因名称 AIDA
Gene ID (NCBI) 64853
RRIDAB_2923676
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96BJ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.

实验方案

Product Specific Protocols
WB protocol for AIDA antibody 68153-1-IgDownload protocol
IF protocol for AIDA antibody 68153-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols