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AIDA Monoclonal antibody

AIDA Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11A1

Cat no : 68153-1-Ig

Print datasheet

Synonyms

AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7

验证数据展示

  • WB analysis using 68153-1-Ig

    WB analysis using 68153-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68153-1-Ig

    WB analysis using 68153-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68153-1-Ig

    WB analysis of HepG2 using 68153-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 68153-1-Ig

    IF Staining of A431 using 68153-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AIDA antibody (68153-1-Ig, Clone: 1D11A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, HepG2 cells, HT-29 cells, U2OS cells, K-562 cells
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68153-1-Ig targets AIDA in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen AIDA fusion protein Ag20597 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name axin interactor, dorsalization associated
Synonyms AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7
Calculated Molecular Weight 306 aa, 35 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC015535
Gene Symbol AIDA
Gene ID (NCBI) 64853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96BJ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.

实验方案

Product Specific Protocols
WB protocol for AIDA antibody 68153-1-IgDownload protocol
IF protocol for AIDA antibody 68153-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols