验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 68153-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68153 targets AIDA in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | AIDA fusion protein Ag20597 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | axin interactor, dorsalization associated |
| 别名 | AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7 |
| 计算分子量 | 306 aa, 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC015535 |
| 基因名称 | AIDA |
| Gene ID (NCBI) | 64853 |
| RRID | AB_3084421 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96BJ3 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AIDA antibody CL488-68153 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |