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CoraLite® Plus 488-conjugated AIDA Monoclonal antibody

AIDA Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D11A1

Cat No : CL488-68153

Print datasheet

Synonyms

AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7

验证数据展示

  • IF Staining of A431 using CL488-68153

    IF Staining of A431 using CL488-68153

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 AIDA antibody (CL488-68153, Clone: 1D11A1 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68153-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68153 targets AIDA in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC
Tested Reactivity Human
Immunogen AIDA fusion protein Ag20597 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name axin interactor, dorsalization associated
Synonyms AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7
Calculated Molecular Weight 306 aa, 35 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC015535
Gene Symbol AIDA
Gene ID (NCBI) 64853
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96BJ3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AIDA antibody CL488-68153Download protocol
Standard Protocols
Click here to view our Standard Protocols