验证数据展示
经过测试的应用
| Positive WB detected in | rabbit brain tissue, mouse retina tissue, rat colon tissue, rat brain tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68154-1-Ig targets DDAH2 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Rat, Mouse, Rabbit |
| 免疫原 | DDAH2 fusion protein Ag7127 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dimethylarginine dimethylaminohydrolase 2 |
| 别名 | DDAH, DDAH 2, DDAH2, DDAHII, Dimethylargininase 2, G6a, NG30, Protein G6a, S phase protein |
| 计算分子量 | 29 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC001435 |
| 基因名称 | DDAH2 |
| Gene ID (NCBI) | 23564 |
| RRID | AB_2923677 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95865 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDAH2 antibody 68154-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |