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验证数据展示

  • WB analysis using 68154-1-Ig (same clone as 68154-1-PBS)

    WB analysis using 68154-1-Ig (same clone as 68154-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse retina using 68154-1-Ig (same clone as 68154-1-PBS)

    WB analysis of mouse retina using 68154-1-Ig (same clone as 68154-1-PBS)

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

  • WB analysis of rat colon using 68154-1-Ig (same clone as 68154-1-PBS)

    WB analysis of rat colon using 68154-1-Ig (same clone as 68154-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50417-2

    Cytometric bead array standard curve of MP50417-2

    Cytometric bead array standard curve of MP50417-2, DDAH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68154-4-PBS. Detection antibody: 68154-1-PBS. Standard:Ag7127. Range: 0.391-50 ng/mL.

DDAH2 Monoclonal antibody, PBS Only (Detector)

DDAH2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 68154-1-PBS

产品说明书

CloneNo. 1A10G3

宿主/亚型

Mouse / IgG3

种属反应性

human, mouse, rat, rabbit

应用

WB, Cytometric bead array, Indirect ELISA

别名

Dimethylargininase 2, DDAHII, DDAH-2, DDAH 2, DDAH

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68154-1-PBS targets DDAH2 as part of a matched antibody pair:

MP50417-2: 68154-4-PBS capture and 68154-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 DDAH2 fusion protein Ag7127 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 dimethylarginine dimethylaminohydrolase 2
别名 Dimethylargininase 2, DDAHII, DDAH-2, DDAH 2, DDAH
计算分子量 29 kDa
观测分子量 30 kDa
GenBank蛋白编号BC001435
基因名称 DDAH2
Gene ID (NCBI) 23564
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95865
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.