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CACYBP Monoclonal antibody

CACYBP Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3C2G11

Cat no : 68161-1-Ig

Print datasheet

Synonyms

CACYBP, calcyclin binding protein, GIG5, hCacyBP, PNAS 107, RP1 102G20.6, S100A6 binding protein, S100A6BP, Siah interacting protein, SIP

验证数据展示

  • WB analysis using 68161-1-Ig

    WB analysis using 68161-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IP experiment of HeLa using 68161-1-Ig

    IP experiment of HeLa using 68161-1-Ig

    IP result of anti-CACYBP (IP:68161-1-Ig, 5ug; Detection:68161-1-Ig 1:10000) with HeLa cells lysate 1880 ug.

  • IF Staining of HEK-293 using 68161-1-Ig

    IF Staining of HEK-293 using 68161-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CACYBP antibody (68161-1-Ig, Clone: 3C2G11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHCT 116 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CACYBP Ready-To-Use IHC Kit. CACYBP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68161-1-Ig targets CACYBP in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CACYBP fusion protein Ag2340 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calcyclin binding protein
Synonyms CACYBP, calcyclin binding protein, GIG5, hCacyBP, PNAS 107, RP1 102G20.6, S100A6 binding protein, S100A6BP, Siah interacting protein, SIP
Calculated Molecular Weight 228 aa, 26 kDa
Observed Molecular Weight26-30 kDa
GenBank Accession NumberBC022352
Gene Symbol CACYBP
Gene ID (NCBI) 27101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HB71
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).

实验方案

Product Specific Protocols
WB protocol for CACYBP antibody 68161-1-IgDownload protocol
IF protocol for CACYBP antibody 68161-1-IgDownload protocol
IP protocol for CACYBP antibody 68161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols