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验证数据展示

  • IF Staining of HEK-293 using CL488-68161

    IF Staining of HEK-293 using CL488-68161

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 CACYBP antibody (CL488-68161, Clone: 3C2G11 ) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-68161

    FC experiment of HEK-293 using CL488-68161

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CACYBP (CL488-68161, Clone:3C2G11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated CACYBP Monoclonal antibody

CACYBP Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68161

Print datasheet

CloneNo. 3C2G11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CACYBP Ready-To-Use IHC Kit. CACYBP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68161-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68161 targets CACYBP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CACYBP fusion protein Ag2340 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calcyclin binding protein
Synonyms Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5
Calculated Molecular Weight 228 aa, 26 kDa
Observed Molecular Weight26-30 kDa
GenBank Accession NumberBC022352
Gene Symbol CACYBP
Gene ID (NCBI) 27101
RRIDAB_3084425
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HB71
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CACYBP antibody CL488-68161Download protocol
FC protocol for CL Plus 488 CACYBP antibody CL488-68161Download protocol
Standard Protocols
Click here to view our Standard Protocols