验证数据展示
经过测试的应用
Positive IF/ICC detected in | HEK-293 cells |
Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68161 targets CACYBP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CACYBP fusion protein Ag2340 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | calcyclin binding protein |
别名 | Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5 |
计算分子量 | 228 aa, 26 kDa |
观测分子量 | 26-30 kDa |
GenBank蛋白编号 | BC022352 |
基因名称 | CACYBP |
Gene ID (NCBI) | 27101 |
RRID | AB_3084425 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9HB71 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CACYBP antibody CL488-68161 | Download protocol |
FC protocol for CL Plus 488 CACYBP antibody CL488-68161 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |