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验证数据展示

  • WB analysis using 68161-1-Ig

    WB analysis using 68161-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IP experiment of HeLa using 68161-1-Ig

    IP experiment of HeLa using 68161-1-Ig

    IP result of anti-CACYBP (IP:68161-1-Ig, 5ug; Detection:68161-1-Ig 1:10000) with HeLa cells lysate 1880 ug.

  • IHC staining of mouse brain using 68161-1-Ig

    IHC staining of mouse brain using 68161-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68161-1-Ig (CACYBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CACYBP antibody included
  • IF Staining of HEK-293 using 68161-1-Ig

    IF Staining of HEK-293 using 68161-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CACYBP antibody (68161-1-Ig, Clone: 3C2G11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

CACYBP Monoclonal antibody

CACYBP Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 68161-1-Ig

Print datasheet

CloneNo. 3C2G11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CACYBP Ready-To-Use IHC Kit. CACYBP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68161-1-Ig targets CACYBP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CACYBP fusion protein Ag2340 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calcyclin binding protein
Synonyms Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5
Calculated Molecular Weight 228 aa, 26 kDa
Observed Molecular Weight26-30 kDa
GenBank Accession NumberBC022352
Gene Symbol CACYBP
Gene ID (NCBI) 27101
RRIDAB_2923680
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HB71
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).

实验方案

Product Specific Protocols
WB protocol for CACYBP antibody 68161-1-IgDownload protocol
IHC protocol for CACYBP antibody 68161-1-IgDownload protocol
IF protocol for CACYBP antibody 68161-1-IgDownload protocol
IP protocol for CACYBP antibody 68161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols