验证数据展示
经过测试的应用
Positive WB detected in | HCT 116 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68161-1-Ig targets CACYBP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CACYBP fusion protein Ag2340 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | calcyclin binding protein |
别名 | Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5 |
计算分子量 | 228 aa, 26 kDa |
观测分子量 | 26-30 kDa |
GenBank蛋白编号 | BC022352 |
基因名称 | CACYBP |
Gene ID (NCBI) | 27101 |
RRID | AB_2923680 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9HB71 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).
实验方案
Product Specific Protocols | |
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WB protocol for CACYBP antibody 68161-1-Ig | Download protocol |
IHC protocol for CACYBP antibody 68161-1-Ig | Download protocol |
IF protocol for CACYBP antibody 68161-1-Ig | Download protocol |
IP protocol for CACYBP antibody 68161-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |