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CACYBP Monoclonal antibody, PBS Only

CACYBP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C2G11

Cat no : 68161-1-PBS

Print datasheet

Synonyms

Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5

验证数据展示

  • WB analysis using 68161-1-Ig (same clone as 68161-1-PBS)

    WB analysis using 68161-1-Ig (same clone as 68161-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68161-1-Ig (same clone as 68161-1-PBS)

    IP experiment of HeLa using 68161-1-Ig (same clone as 68161-1-PBS)

    IP result of anti-CACYBP (IP:68161-1-Ig, 5ug; Detection:68161-1-Ig 1:10000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68161-1-Ig (same clone as 68161-1-PBS)

    IHC staining of mouse brain using 68161-1-Ig (same clone as 68161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68161-1-Ig (CACYBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CACYBP antibody included
  • IF Staining of HEK-293 using 68161-1-Ig (same clone as 68161-1-PBS)

    IF Staining of HEK-293 using 68161-1-Ig (same clone as 68161-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CACYBP antibody (68161-1-Ig, Clone: 3C2G11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CACYBP Ready-To-Use IHC Kit. CACYBP primary antibody included.

产品信息

68161-1-PBS targets CACYBP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CACYBP fusion protein Ag2340 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calcyclin binding protein
Synonyms Calcyclin, S100 alpha 6, 3C2G11, hCacyBP, GIG5
Calculated Molecular Weight 228 aa, 26 kDa
Observed Molecular Weight26-30 kDa
GenBank Accession NumberBC022352
Gene Symbol CACYBP
Gene ID (NCBI) 27101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HB71
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).