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验证数据展示

  • WB analysis using 68163-1-Ig

    WB analysis using 68163-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 68163-1-Ig

    WB analysis using 68163-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of rat brain using 68163-1-Ig

    IP experiment of rat brain using 68163-1-Ig

    IP result of anti-PPP3CA (IP:68163-1-Ig, 5ug; Detection:68163-1-Ig 1:4000) with rat brain tissue lysate 1120 ug.

  • IHC staining of human thyroid cancer using 68163-1-Ig

    IHC staining of human thyroid cancer using 68163-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP3CA antibody included
  • IHC staining of human thyroid cancer using 68163-1-Ig

    IHC staining of human thyroid cancer using 68163-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP3CA antibody included

PPP3CA Monoclonal antibody

PPP3CA Monoclonal Antibody for WB, IHC, IP, ELISA
Cat No. 68163-1-Ig

Print datasheet

CloneNo. 2A5C1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IP, ELISA

Synonyms

CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, rabbit skeletal muscle tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, rat skeletal muscle
Positive IP detected inrat brain tissue
Positive IHC detected inhuman thyroid cancer tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPP3CA Ready-To-Use IHC Kit. PPP3CA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68163-1-Ig targets PPP3CA in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen PPP3CA fusion protein Ag32642 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
Synonyms CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha
Calculated Molecular Weight 59 kDa
Observed Molecular Weight59 kDa
GenBank Accession NumberBC025714
Gene Symbol PPP3CA
Gene ID (NCBI) 5530
RRIDAB_2923682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08209
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.

实验方案

Product Specific Protocols
WB protocol for PPP3CA antibody 68163-1-IgDownload protocol
IHC protocol for PPP3CA antibody 68163-1-IgDownload protocol
IP protocol for PPP3CA antibody 68163-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols