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验证数据展示

  • WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 68163-1-Ig (same clone as 68163-1-PBS)

    IP experiment of rat brain using 68163-1-Ig (same clone as 68163-1-PBS)

    IP result of anti-PPP3CA (IP:68163-1-Ig, 5ug; Detection:68163-1-Ig 1:4000) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPP3CA antibody included
  • IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPP3CA antibody included

PPP3CA Monoclonal antibody, PBS Only

PPP3CA Monoclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 68163-1-PBS

产品说明书

CloneNo. 2A5C1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IP, Indirect ELISA

别名

CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PPP3CA Ready-To-Use IHC Kit. PPP3CA primary antibody included.

产品信息

68163-1-PBS targets PPP3CA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 PPP3CA fusion protein Ag32642 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
别名 CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha
计算分子量 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC025714
基因名称 PPP3CA
Gene ID (NCBI) 5530
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ08209
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.