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PPP3CA Monoclonal antibody, PBS Only

PPP3CA Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5C1

Cat no : 68163-1-PBS

Print datasheet

Synonyms

CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha

验证数据展示

  • WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 68163-1-Ig (same clone as 68163-1-PBS)

    IP experiment of rat brain using 68163-1-Ig (same clone as 68163-1-PBS)

    IP result of anti-PPP3CA (IP:68163-1-Ig, 5ug; Detection:68163-1-Ig 1:4000) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPP3CA antibody included
  • IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPP3CA antibody included
Planning an IHC experiment? We recommend our IHCeasy PPP3CA Ready-To-Use IHC Kit. PPP3CA primary antibody included.

产品信息

68163-1-PBS targets PPP3CA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen PPP3CA fusion protein Ag32642 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
Synonyms CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha
Calculated Molecular Weight 59 kDa
Observed Molecular Weight59 kDa
GenBank Accession NumberBC025714
Gene Symbol PPP3CA
Gene ID (NCBI) 5530
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08209
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.