验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, K-562 cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68165-1-Ig targets USP36 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | USP36 fusion protein Ag6426 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 36 |
| 别名 | Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1, Deubiquitinating enzyme 36 |
| 计算分子量 | 1123 aa, 123 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC038983 |
| 基因名称 | USP36 |
| Gene ID (NCBI) | 57602 |
| RRID | AB_2923684 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9P275 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP36 antibody 68165-1-Ig | Download protocol |
| IF protocol for USP36 antibody 68165-1-Ig | Download protocol |
| IP protocol for USP36 antibody 68165-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |