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验证数据展示

  • IF Staining of A549 using 68175-1-Ig

    IF Staining of A549 using 68175-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using BLVRA antibody (68175-1-Ig, Clone: 2A10F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • WB analysis using 68175-1-Ig

    WB analysis using 68175-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68175-1-Ig (BLVRA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

BLVRA Monoclonal antibody

BLVRA Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68175-1-Ig

产品说明书

CloneNo. 2A10F2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

BVR A, BVR, BLVR, Biliverdin-IX alpha-reductase, biliverdin reductase A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, DU 145 cells, U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68175-1-Ig targets BLVRA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 BLVRA fusion protein Ag32730 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 biliverdin reductase A
别名 BVR A, BVR, BLVR, Biliverdin-IX alpha-reductase, biliverdin reductase A
计算分子量33.2 kDa
观测分子量 37-40 kDa, 66 kDa
GenBank蛋白编号BC008456
基因名称 BLVRA
Gene ID (NCBI) 644
RRIDAB_2935266
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP53004
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BLVRA(Biliverdin reductase A) is also named BLVR, BVR, and belongs to the biliverdin reductase subfamily. It catalyzes the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. This protein can exist as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).

实验方案

Product Specific Protocols
WB protocol for BLVRA antibody 68175-1-IgDownload protocol
IF protocol for BLVRA antibody 68175-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols