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DFFA,DFF45 Monoclonal antibody

DFFA,DFF45 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F6H9

Cat no : 68177-1-Ig

Print datasheet

Synonyms

DFF45, DFFA, DFF45/DFFA, DFF-45, DFF1

验证数据展示

  • WB analysis using 68177-1-Ig

    WB analysis using 68177-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68177-1-Ig (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HEK-293 using 68177-1-Ig

    IF Staining of HEK-293 using 68177-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DFFA,DFF45 antibody (68177-1-Ig, Clone: 1F6H9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HEK-293 using 68177-1-Ig

    FC experiment of HEK-293 using 68177-1-Ig

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug DFFA,DFF45 Monoclonal antibody (68177-1-Ig, Clone:1F6H9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68177-1-Ig targets DFFA,DFF45 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DFFA,DFF45 fusion protein Ag32657 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DNA fragmentation factor, 45kDa, alpha polypeptide
Synonyms DFF45, DFFA, DFF45/DFFA, DFF-45, DFF1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC000037
Gene Symbol DFFA
Gene ID (NCBI) 1676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.

实验方案

Product Specific Protocols
WB protocol for DFFA,DFF45 antibody 68177-1-IgDownload protocol
IF protocol for DFFA,DFF45 antibody 68177-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols