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验证数据展示

  • WB analysis using 68179-1-Ig

    WB analysis using 68179-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68179-1-Ig (ENOPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • FC experiment of HepG2 using 68179-1-Ig

    FC experiment of HepG2 using 68179-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ENOPH1 Monoclonal antibody (68179-1-Ig, Clone:1F1E11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ENOPH1 Monoclonal antibody

ENOPH1 Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 68179-1-Ig

产品说明书

CloneNo. 1F1E11

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Rabbit, Pig

应用

WB, ELISA, FC (Intra)

别名

MASA, Enolase-phosphatase E1, EC:3.1.3.77, E1, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68179-1-Ig targets ENOPH1 in WB, FC (Intra), ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat, Rabbit, Pig
免疫原 ENOPH1 fusion protein Ag12553 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 enolase-phosphatase 1
别名 MASA, Enolase-phosphatase E1, EC:3.1.3.77, E1, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
计算分子量 261 aa, 29 kDa
观测分子量29-35 kDa
GenBank蛋白编号BC065815
基因名称 ENOPH1
Gene ID (NCBI) 58478
RRIDAB_2935270
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UHY7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.

实验方案

Product Specific Protocols
WB protocol for ENOPH1 antibody 68179-1-IgDownload protocol
FC protocol for ENOPH1 antibody 68179-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols