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APPL1 Monoclonal antibody

APPL1 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B7B11

Cat No : 68195-1-Ig

Print datasheet

Synonyms

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428

验证数据展示

  • WB analysis using 68195-1-Ig

    WB analysis using 68195-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68195-1-Ig

    WB analysis using 68195-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68195-1-Ig

    WB analysis using 68195-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68195-1-Ig

    WB analysis of HepG2 using 68195-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig heart using 68195-1-Ig

    WB analysis of pig heart using 68195-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 68195-1-Ig

    WB analysis of HEK-293 using 68195-1-Ig

    WB result of APPL1 antibody (68195-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.

  • IHC staining of human colon cancer using 68195-1-Ig

    IHC staining of human colon cancer using 68195-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68195-1-Ig (APPL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APPL1 antibody included
  • IF Staining of HepG2 using 68195-1-Ig

    IF Staining of HepG2 using 68195-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (68195-1-Ig, Clone: 1B7B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HepG2 using 68195-1-Ig

    FC experiment of HepG2 using 68195-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (68195-1-Ig, Clone:1B7B11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, rabbit heart tissue, HepG2 cells, pig heart tissue, HEK-293 cells, HeLa cells, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APPL1 Ready-To-Use IHC Kit. APPL1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68195-1-Ig targets APPL1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen APPL1 fusion protein Ag3334 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Synonyms APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428
Calculated Molecular Weight 709 aa, 80 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC028599
Gene Symbol APPL1
Gene ID (NCBI) 26060
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKG1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

实验方案

Product Specific Protocols
WB protocol for APPL1 antibody 68195-1-IgDownload protocol
IHC protocol for APPL1 antibody 68195-1-IgDownload protocol
IF protocol for APPL1 antibody 68195-1-IgDownload protocol
FC protocol for APPL1 antibody 68195-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols