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验证数据展示

  • WB analysis using 68195-1-Ig (same clone as 68195-1-PBS)

    WB analysis using 68195-1-Ig (same clone as 68195-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • WB analysis using 68195-1-Ig (same clone as 68195-1-PBS)

    WB analysis using 68195-1-Ig (same clone as 68195-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • WB analysis using 68195-1-Ig (same clone as 68195-1-PBS)

    WB analysis using 68195-1-Ig (same clone as 68195-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68195-1-Ig (same clone as 68195-1-PBS)

    WB analysis of HepG2 using 68195-1-Ig (same clone as 68195-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • WB analysis of pig heart using 68195-1-Ig (same clone as 68195-1-PBS)

    WB analysis of pig heart using 68195-1-Ig (same clone as 68195-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 68195-1-Ig (same clone as 68195-1-PBS)

    WB analysis of HEK-293 using 68195-1-Ig (same clone as 68195-1-PBS)

    WB result of APPL1 antibody (68195-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 68195-1-Ig (same clone as 68195-1-PBS)

    IHC staining of human colon cancer using 68195-1-Ig (same clone as 68195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68195-1-Ig (APPL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APPL1 antibody included
  • IF Staining of HepG2 using 68195-1-Ig (same clone as 68195-1-PBS)

    IF Staining of HepG2 using 68195-1-Ig (same clone as 68195-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (68195-1-Ig, Clone: 1B7B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68195-1-Ig (same clone as 68195-1-PBS)

    FC experiment of HepG2 using 68195-1-Ig (same clone as 68195-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (68195-1-Ig, Clone:1B7B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

APPL1 Monoclonal antibody, PBS Only

APPL1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68195-1-PBS

产品说明书

CloneNo. 1B7B11

宿主/亚型

Mouse / IgG2b

种属反应性

rabbit, pig, rat, mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

DIP13A, Dip13 alpha, DCC-interacting protein 13-alpha, APPL, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy APPL1 Ready-To-Use IHC Kit. APPL1 primary antibody included.

产品信息

68195-1-PBS targets APPL1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 rabbit, pig, rat, mouse, human
免疫原 APPL1 fusion protein Ag3334 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
别名 DIP13A, Dip13 alpha, DCC-interacting protein 13-alpha, APPL, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
计算分子量 709 aa, 80 kDa
观测分子量80 kDa
GenBank蛋白编号BC028599
基因名称 APPL1
Gene ID (NCBI) 26060
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UKG1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].