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验证数据展示

  • WB analysis using 68209-1-Ig (same clone as 68209-1-PBS)

    WB analysis using 68209-1-Ig (same clone as 68209-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68209-1-Ig (DNM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68209-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68209-1-Ig (same clone as 68209-1-PBS)

    IF Staining of HeLa using 68209-1-Ig (same clone as 68209-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNM2 antibody (68209-1-Ig, Clone: 1B8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68209-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50954-1

    Cytometric bead array standard curve of MP50954-1

    Cytometric bead array standard curve of MP50954-1, DNM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68209-1-PBS. Detection antibody: 68209-2-PBS. Standard:Ag6274. Range: 0.781-100 ng/mL.

DNM2 Monoclonal antibody, PBS Only (Capture)

DNM2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68209-1-PBS

产品说明书

CloneNo. 1B8H3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

dynamin2, Dynamin II, dynamin 2, DYN2, DNM 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68209-1-PBS targets DNM2 as part of a matched antibody pair:

MP50954-1: 68209-1-PBS capture and 68209-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 DNM2 fusion protein Ag6274 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dynamin 2
别名 dynamin2, Dynamin II, dynamin 2, DYN2, DNM 2
计算分子量 98 kDa
观测分子量 100 kDa
GenBank蛋白编号BC054501
基因名称 DNM2
Gene ID (NCBI) 1785
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50570
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.