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验证数据展示

  • WB analysis using 68209-1-Ig

    WB analysis using 68209-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68209-1-Ig (DNM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of HeLa using 68209-1-Ig

    IF Staining of HeLa using 68209-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNM2 antibody (68209-1-Ig, Clone: 1B8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DNM2 Monoclonal antibody

DNM2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68209-1-Ig

Print datasheet

CloneNo. 1B8H3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

CMTDI1, CMTDIB, DI CMTB, DNM2, DYN2, dynamin 2, DYNII

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®555, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68209-1-Ig targets DNM2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen DNM2 fusion protein Ag6274 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dynamin 2
Synonyms CMTDI1, CMTDIB, DI CMTB, DNM2, DYN2, dynamin 2, DYNII
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC054501
Gene Symbol DNM2
Gene ID (NCBI) 1785
RRIDAB_2935297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP50570
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.

实验方案

Product Specific Protocols
WB protocol for DNM2 antibody 68209-1-IgDownload protocol
IF protocol for DNM2 antibody 68209-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols