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验证数据展示

  • WB analysis using 68209-1-Ig

    WB analysis using 68209-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68209-1-Ig (DNM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of HeLa using 68209-1-Ig

    IF Staining of HeLa using 68209-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNM2 antibody (68209-1-Ig, Clone: 1B8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DNM2 Monoclonal antibody

DNM2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68209-1-Ig

产品说明书

CloneNo. 1B8H3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

CMTDI1, CMTDIB, DI CMTB, DNM2, DYN2, dynamin 2, DYNII

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®555, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68209-1-Ig targets DNM2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 DNM2 fusion protein Ag6274 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dynamin 2
别名 CMTDI1, CMTDIB, DI CMTB, DNM2, DYN2, dynamin 2, DYNII
计算分子量 98 kDa
观测分子量 100 kDa
GenBank蛋白编号BC054501
基因名称 DNM2
Gene ID (NCBI) 1785
RRIDAB_2935297
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP50570
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.

实验方案

Product Specific Protocols
WB protocol for DNM2 antibody 68209-1-IgDownload protocol
IF protocol for DNM2 antibody 68209-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols