验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-68209 targets DNM2 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat, pig, rabbit |
| Immunogen | DNM2 fusion protein Ag6274 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | dynamin 2 |
| Synonyms | CMTDI1, CMTDIB, DI CMTB, DNM2, DYN2, dynamin 2, DYNII |
| Calculated Molecular Weight | 98 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC054501 |
| Gene Symbol | DNM2 |
| Gene ID (NCBI) | 1785 |
| RRID | AB_3084829 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P50570 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 DNM2 antibody CL647-68209 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |