CoraLite® Plus 647-conjugated DNM2 Monoclonal antibody

DNM2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1B8H3

Cat no : CL647-68209

Print datasheet

Synonyms

CMTDI1, CMTDIB, DI CMTB, DNM2, DYN2, dynamin 2, DYNII



经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68209-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-68209 targets DNM2 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen DNM2 fusion protein Ag6274 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dynamin 2
Synonyms CMTDI1, CMTDIB, DI CMTB, DNM2, DYN2, dynamin 2, DYNII
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC054501
Gene Symbol DNM2
Gene ID (NCBI) 1785
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP50570
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 DNM2 antibody CL647-68209Download protocol
Standard Protocols
Click here to view our Standard Protocols