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GSTM5 Monoclonal antibody

GSTM5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E5C2

Cat no : 68210-1-Ig

Print datasheet

Synonyms

glutathione S transferase mu 5, GST class mu 5, GSTM5, GSTM5 5, GTM5

验证数据展示

  • WB analysis using 68210-1-Ig

    WB analysis using 68210-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68210-1-Ig

    WB analysis using 68210-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig liver using 68210-1-Ig

    WB analysis of pig liver using 68210-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrabbit brain tissue, pig liver tissue, rabbit liver tissue, rat brain tissue, mouse brain tissue, mouse liver tissue, mouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68210-1-Ig targets GSTM5 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat, pig, rabbit
Cited Reactivityhuman
Immunogen GSTM5 fusion protein Ag6054 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glutathione S-transferase mu 5
Synonyms glutathione S transferase mu 5, GST class mu 5, GSTM5, GSTM5 5, GTM5
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC058881
Gene Symbol GSTM5
Gene ID (NCBI) 2949
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46439
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTM5, Glutathione S-transferases, belongs to eukaryotic and prokaryotic phase II metabolic isozymes family. It can catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for detoxification. GSTM5 undergo epigenetic repression in AMD (age-related macular degeneration) RPE/choroid, which may increase susceptibility to oxidative stress in AMD retinas (PMID: 22410570). GSTM5 was found in brain, lung and testis (PMID: 9230131).

实验方案

Product Specific Protocols
WB protocol for GSTM5 antibody 68210-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Lasers Med Sci

Revealing the mechanism of 755-nm long-pulsed alexandrite laser in inhibiting infantile hemangioma endothelial cells through transcriptome sequencing

Authors - Chen Ke
View Article