CD163 Monoclonal antibody
CD163 Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2G7A5
验证数据展示
经过测试的应用
Positive WB detected in | human placenta tissue |
Positive IHC detected in | human liver tissue, human placenta tissue, human tonsillitis tissue, human lung cancer tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human liver tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
68218-1-Ig targets CD163 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF-P, IHC, WB, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | Human |
Cited Reactivity | human |
Immunogen | CD163 fusion protein Ag26721 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | CD163 molecule |
Synonyms | CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163 |
Calculated Molecular Weight | 1156 aa, 125 kDa |
Observed Molecular Weight | 145 kDa |
GenBank Accession Number | BC051281 |
Gene Symbol | CD163 |
Gene ID (NCBI) | 9332 |
ENSEMBL Gene ID | ENSG00000177575 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q86VB7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD163 antibody 68218-1-Ig | Download protocol |
IHC protocol for CD163 antibody 68218-1-Ig | Download protocol |
IF protocol for CD163 antibody 68218-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Immunol Immunother A comprehensive pan-cancer analysis of prognostic value and potential clinical implications of FTH1 in cancer immunotherapy | ||
iScience Independent prognostic value of CLDN6 in bladder cancer based on M2 macrophages related signature |