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CD163 Monoclonal antibody, PBS Only

CD163 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2G7A5

Cat No : 68218-1-PBS

Print datasheet

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

验证数据展示

  • WB analysis of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

    WB analysis of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68218-1-Ig (CD163 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

    IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD163 antibody included
  • IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

    IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD163 antibody included
  • IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

    IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

    IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.

产品信息

68218-1-PBS targets CD163 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen CD163 fusion protein Ag26721 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD163 molecule
Synonyms CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163
Calculated Molecular Weight 1156 aa, 125 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86VB7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.