CD163 Monoclonal antibody

CD163 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 68218-1-Ig

产品说明书

CloneNo. 2G7A5

宿主/亚型

Mouse / IgG1

种属反应性

human and More (2)

应用

WB, IHC, IF-P, IP, ELISA and More (1)

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, CD163 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman liver tissue, human appendicitis tissue, human lung cancer tissue, human placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68218-1-Ig targets CD163 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse, rabbit
免疫原 CD163 fusion protein Ag26721 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD163 molecule
别名 Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, CD163 molecule
计算分子量 1156 aa, 125 kDa
观测分子量145-150 kDa
GenBank蛋白编号BC051281
基因名称 CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
RRIDAB_2935306
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ86VB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

实验方案

Product Specific Protocols
WB protocol for CD163 antibody 68218-1-IgDownload protocol
IHC protocol for CD163 antibody 68218-1-IgDownload protocol
IF protocol for CD163 antibody 68218-1-IgDownload protocol
IP protocol for CD163 antibody 68218-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Immunol Immunother

A comprehensive pan-cancer analysis of prognostic value and potential clinical implications of FTH1 in cancer immunotherapy

Authors - Yuejun Luo
humanWB

iScience

Independent prognostic value of CLDN6 in bladder cancer based on M2 macrophages related signature

Authors - Da Qi
rabbitWB,IF

Biomaterials

Multilayered hydrogel scaffold construct with native tissue matched elastic modulus: A regenerative microenvironment for urethral scar-free healing

Authors - Yangwang Jin
mouseIHC,IF

Am J Cancer Res

STAC3 as a poor prognostic biomarker in renal clear cell carcinoma: relationship with immune infiltration

Authors - Yingnan Zhang
humanIHC,IF

Int J Biol Sci

Tumor-associated macrophages drive heterogenetic CD10High cancer stem cells to implement tumor-associated neutrophils reprogramming in oral squamous cell carcinoma

Authors - Yuanhe You
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