• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68219-1-Ig

    WB analysis using 68219-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68219-1-Ig (ATP6V1B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 68219-1-Ig

    IF Staining of HEK-293 using 68219-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (68219-1-Ig, Clone: 1G10A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

ATP6V1B1 Monoclonal antibody

ATP6V1B1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68219-1-Ig

产品说明书

CloneNo. 1G10A8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Rabbit, Pig

应用

WB, IF/ICC, ELISA

别名

ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJAR cells, pig brain tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68219-1-Ig targets ATP6V1B1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Rabbit, Pig
免疫原 ATP6V1B1 fusion protein Ag7405 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
别名 ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3
计算分子量 57 kDa
观测分子量56 kDa
GenBank蛋白编号BC063411
基因名称 ATP6V1B1
Gene ID (NCBI) 525
RRIDAB_2935307
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP15313
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

实验方案

Product Specific Protocols
WB protocol for ATP6V1B1 antibody 68219-1-IgDownload protocol
IF protocol for ATP6V1B1 antibody 68219-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols